6Q58

Copper loading to a cytosolic copper storage protein from Streptomyces lividans (five coppers)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.33
Spacegroup name	P 61 2 2
Unit cell lengths	93.051, 93.051, 212.340
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	212.340 - 1.500
R-factor	0.1955
Rwork	0.195
R-free	0.21350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ei0
RMSD bond length	0.013
RMSD bond angle	1.472
Data reduction software	DIALS
Data scaling software	Aimless (0.6.2)
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	212.340	75.340	1.530
High resolution limit [Å]	1.500	8.220	1.500
Rmerge	0.057	0.054	0.659
Rmeas	0.060	0.058	0.801
Rpim	0.020	0.018	0.447
Number of reflections	85587	628	3492
<I/σ(I)>	19.5
Completeness [%]	98.0	96.2	82.5
Redundancy	8	8.7	2.9
CC(1/2)	0.999	0.994	0.448

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.5 M Ammonium Sulfate 0.1M MES pH 6.0