6Q4V
KlenTaq DNA polymerase in complex with dATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 109.482, 109.482, 91.255 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.407 - 2.006 |
| R-factor | 0.1874 |
| Rwork | 0.186 |
| R-free | 0.21860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rtv |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.623 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_3283: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.407 | 2.016 |
| High resolution limit [Å] | 2.006 | 2.006 |
| Rmeas | 0.076 | 1.082 |
| Number of reflections | 81683 | 12970 |
| <I/σ(I)> | 11.59 | 1.53 |
| Completeness [%] | 99.6 | 97.8 |
| Redundancy | 5.2 | 5.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 27 % PEG 4000, 0.2 M ammonium acetate, 0.01 M magnesium (II) acetate, 0.1 M MES pH 6.5 |
