6Q3M
Structure of CHD4 PHD2 - tandem chromodomains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-08-01 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.731, 87.681, 129.257 |
Unit cell angles | 90.00, 93.57, 90.00 |
Refinement procedure
Resolution | 64.503 - 2.520 |
R-factor | 0.2315 |
Rwork | 0.230 |
R-free | 0.26260 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.801 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.503 | 2.610 |
High resolution limit [Å] | 2.520 | 2.520 |
Number of reflections | 106090 | 5220 |
<I/σ(I)> | 7.98 | |
Completeness [%] | 99.3 | |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | 0.12M Diethyleneglycol, 0.12M Triethylene-glycol, 0.12M Tetraethyleneglycol, 0.12M Pentaethyleneglycol, 20% v/v Ethylene glycol, 10 % w/v PEG 8000, Sodium HEPES, MOPS pH = 7.5 |