6Q39

Complex of Arginase 2 with Example 49

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-08
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54178
Spacegroup name	P 42 21 2
Unit cell lengths	128.303, 128.303, 159.155
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.912 - 2.213
R-factor	0.1647
Rwork	0.162
R-free	0.20980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1d3v
RMSD bond length	0.008
RMSD bond angle	1.105
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.210
Rmerge	0.126	0.447
Total number of observations	589289
Number of reflections	61688	6783
<I/σ(I)>	16.1	4.7
Completeness [%]	91.3	55.4
Redundancy	9.6	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	CRYSTALS IN CONDITION H3 FROM SILVER BULLET SCREEN (HAMPTON RESEARCH) USING TACSIMATE IN THE