6Q2L
The structure of the Streptococcus gordonii surface protein SspB in complex with TEV peptide provides clues to the adherence of oral streptococcal adherence to salivary agglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-30 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.223, 47.695, 64.652 |
| Unit cell angles | 90.00, 113.60, 90.00 |
Refinement procedure
| Resolution | 37.150 - 1.800 |
| R-factor | 0.197 |
| Rwork | 0.195 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6q2k |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.299 |
| Data reduction software | HKL-2000 (2.3.8) |
| Data scaling software | HKL-2000 (2.3.8) |
| Phasing software | PHASER (2.7.17) |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.084 | 0.419 |
| Rmeas | 0.097 | 0.567 |
| Rpim | 0.047 | 0.380 |
| Number of reflections | 31075 | 1321 |
| <I/σ(I)> | 11.8 | 1.6 |
| Completeness [%] | 98.9 | 83.9 |
| Redundancy | 3.3 | 1.5 |
| CC(1/2) | 0.714 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20-25% PEG 8000, 0.1M succinic acid, pH 5.0-5.5, 100mM MgCl2 |
