6Q25
Coiled-coil Trimer with Glu:Leu:Lys Triad
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-09-29 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.5406 |
| Spacegroup name | H 3 |
| Unit cell lengths | 39.063, 39.063, 101.522 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 17.409 - 1.893 |
| R-factor | 0.1818 |
| Rwork | 0.176 |
| R-free | 0.22790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_2906) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.409 | 1.961 |
| High resolution limit [Å] | 1.893 | 1.893 |
| Rmerge | 0.191 | 0.532 |
| Rmeas | 0.211 | 0.658 |
| Rpim | 0.087 | 0.380 |
| Number of reflections | 4584 | 469 |
| <I/σ(I)> | 9.9 | 1.4 |
| Completeness [%] | 98.9 | |
| Redundancy | 4.9 | |
| CC(1/2) | 0.948 | 0.668 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 50% PEG200, 100 mM HEPES free acid/NaOH, pH 7.5 |
