6Q23
Crystal structure of human 1G01 Fab in complex with influenza virus neuraminidase from A/California/04/2009 (H1N1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.03309 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.594, 234.985, 106.275 |
| Unit cell angles | 90.00, 106.40, 90.00 |
Refinement procedure
| Resolution | 43.300 - 3.270 |
| R-factor | 0.19841 |
| Rwork | 0.196 |
| R-free | 0.24078 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nss 5itb 4fql |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.384 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.300 | 3.380 |
| High resolution limit [Å] | 3.270 | 3.270 |
| Rpim | 0.120 | 0.430 |
| Number of reflections | 70214 | 3688 |
| <I/σ(I)> | 4.5 | 1.1 |
| Completeness [%] | 91.9 | 73.1 |
| Redundancy | 2.7 | 2.2 |
| CC(1/2) | 0.952 | 0.876 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Tris, pH 8.5, 10% (v/v) glycerol, 20% (w/v) PEG300, 5% (w/v) PEG8000 |
