6Q1J

Antibody H2227 from the human antibody lineage 652

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	80
Detector technology	CCD
Collection date	2014-10-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	57.300, 69.380, 142.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.691 - 2.703
R-factor	0.2263
Rwork	0.225
R-free	0.24560
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.691	44.180
High resolution limit [Å]	2.700	2.700
Rmerge	0.139	0.970
Number of reflections	15536	1530
<I/σ(I)>	13.65
Completeness [%]	95.8	95.98
Redundancy	5.6
CC(1/2)	0.998	0.703

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	291.15	100mM ammonium sulfate, 40% (v/v) PEG 400, 100mM sodium citrate pH 4.0