6Q1I

GH5-4 broad specificity endoglucanase from Clostrdium longisporum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-13
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.978560
Spacegroup name	P 1
Unit cell lengths	45.350, 53.480, 68.060
Unit cell angles	104.05, 96.89, 87.06

Refinement procedure

Resolution	25.210 - 1.350
R-factor	0.1119
Rwork	0.112
R-free	0.13170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ndy
RMSD bond length	0.005
RMSD bond angle	0.850
Data reduction software	XDS (20190315)
Data scaling software	XSCALE (20190315)
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.210	1.398
High resolution limit [Å]	1.350	1.350
Rmerge	0.040	0.108
Rmeas	0.047	0.130
Rpim	0.024	0.071
Number of reflections	128382	12528
<I/σ(I)>	20.86	8.28
Completeness [%]	94.8
Redundancy	3.8
CC(1/2)	0.998	0.988

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	Crystals were set with a Mosquito into SD2 plates. The condition that provided the crystals for this data set came from Morpheus Screen D9: 10% Alcohols, 50% Tris/Bicine H 8.5, and 15% P550MME_P20K