6Q1G

Human antibody H1244 of the human antibody lineage 652

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	80
Detector technology	CCD
Collection date	2017-09-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	67.140, 67.470, 95.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.612 - 1.750
R-factor	0.1809
Rwork	0.180
R-free	0.20640
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.612	1.813
High resolution limit [Å]	1.750	1.750
Rmerge	0.068	1.225
Number of reflections	44531	4380
<I/σ(I)>	20.5
Completeness [%]	99.9	99.91
Redundancy	8
CC(1/2)	0.999	0.660

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	100mM Potassium thiocyanate, 30% (w/v) PEG 2000 MME