6Q1E

Inferred precursor (UCA) of the human antibody lineage 652

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	80
Detector technology	CCD
Collection date	2014-12-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	45.440, 69.670, 74.440
Unit cell angles	90.00, 100.08, 90.00

Refinement procedure

Resolution	44.738 - 2.500
R-factor	0.2245
Rwork	0.223
R-free	0.25740
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.738	2.589
High resolution limit [Å]	2.500	2.500
Rmerge	0.132	1.031
Number of reflections	15923	1576
<I/σ(I)>	10.85
Completeness [%]	99.7
Redundancy	3.7
CC(1/2)	0.993	0.579

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291.15	5% (v/v) PEG 400, 1.4M sodium citrate pH 5.0