6Q19

Inferred intermediate (I-6) of the human antibody lineage 652

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	80
Detector technology	CCD
Collection date	2016-05-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9998
Spacegroup name	P 1 21 1
Unit cell lengths	47.770, 70.030, 65.650
Unit cell angles	90.00, 108.21, 90.00

Refinement procedure

Resolution	46.573 - 1.580
R-factor	0.1652
Rwork	0.164
R-free	0.19100
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.573	1.637
High resolution limit [Å]	1.580	1.580
Rmerge	0.061	0.671
Number of reflections	55518	4982
<I/σ(I)>	14.25
Completeness [%]	98.5	89.19
Redundancy	3.5
CC(1/2)	0.999	0.517

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291.15	1M sodium chloride, 30% (w/v) PEG 8000, 100 mM HEPES pH7.0