6Q0H

Inferred intermediate I-7 (I-7-0) of the human antibody lineage 652 in complex with influenza hemagglutinin head domain of A/Beijing/262/95(H1N1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	80
Detector technology	CCD
Collection date	2018-06-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	52.810, 70.580, 200.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.585 - 2.750
R-factor	0.2426
Rwork	0.241
R-free	0.26920
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.585	2.848
High resolution limit [Å]	2.750	2.750
Rmerge	0.069	0.951
Number of reflections	20295	1980
<I/σ(I)>	23.15	2.2
Completeness [%]	100.0	100
Redundancy	8.6	9
CC(1/2)	0.999	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291.15	5% (v/v) PEG 400, 1.1M sodium citrate