6PZD
Crystal structure of the neuraminidase stabilization mutant Y169aH from A/Shanghai/2/2013 (H7N9)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-21 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | I 4 3 2 |
Unit cell lengths | 182.219, 182.219, 182.219 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.949 - 1.120 |
R-factor | 0.113 |
Rwork | 0.112 |
R-free | 0.12800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5l14 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.171 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.000 | 1.130 |
High resolution limit [Å] | 1.120 | 1.120 |
Rpim | 0.020 | 0.450 |
Number of reflections | 194141 | 6246 |
<I/σ(I)> | 30.8 | 1.1 |
Completeness [%] | 99.9 | |
Redundancy | 22.3 | |
CC(1/2) | 0.999 | 0.834 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MES pH 6.5, 12% PEG20000 |