6PXS

Crystal structure of iminodiacetate oxidase (IdaA) from Chelativorans sp. BNC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-08
Detector	PSI PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	113.457, 113.457, 266.119
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.842 - 2.836
R-factor	0.2191
Rwork	0.217
R-free	0.24940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ryi
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.850
High resolution limit [Å]	2.800	7.590	2.800
Rmerge	0.479	0.055	5.985
Rmeas	0.499	0.057	6.834
Rpim	0.137	0.016	3.199
Total number of observations	548682
Number of reflections	43608	2434	2066
<I/σ(I)>	2.7
Completeness [%]	99.8	100	96.2
Redundancy	12.6	13.2	4
CC(1/2)		0.999	0.044

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M tris pH 8.5, 0.2 M trimethylamine N-oxide dihydrate, 20% (w/v) PEG MME 2,000