6PXN

Human Casein Kinase 1 delta Tau mutant (R178C)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-18
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0332
Spacegroup name	P 1 21 1
Unit cell lengths	50.081, 129.640, 51.860
Unit cell angles	90.00, 113.41, 90.00

Refinement procedure

Resolution	44.676 - 1.551
R-factor	0.1783
Rwork	0.177
R-free	0.20500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5x17
RMSD bond length	0.014
RMSD bond angle	1.373
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.820	1.580
High resolution limit [Å]	1.550	1.550
Rmerge	0.063	0.601
Rpim	0.028	0.270
Number of reflections	85497	4108
<I/σ(I)>	13.1
Completeness [%]	97.8	95.3
Redundancy	6.9	6.7
CC(1/2)	0.990	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	295	0.3 M Ammonium sulfate 0.05 M Sodium Acetate 18% PEG 2000