6PXK
3.65 Angstroms resolution structure of HslU with an axial-channel plug
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-03 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 200.030, 91.200, 201.800 |
| Unit cell angles | 90.00, 99.43, 90.00 |
Refinement procedure
| Resolution | 47.687 - 3.647 |
| R-factor | 0.2317 |
| Rwork | 0.230 |
| R-free | 0.27050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1do0 |
| Data reduction software | HKL-2000 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.687 | 47.687 | 3.740 |
| High resolution limit [Å] | 3.647 | 16.310 | 3.650 |
| Rmerge | 0.105 | 0.042 | 1.287 |
| Rmeas | 0.117 | 0.047 | 1.440 |
| Number of reflections | 154279 | 1652 | 10641 |
| <I/σ(I)> | 8.9 | 30.18 | 1.02 |
| Completeness [%] | 98.4 | 92.9 | 92.5 |
| Redundancy | 5.01 | 4.85 | 4.689 |
| CC(1/2) | 0.998 | 0.997 | 0.609 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M ammonium sulfate, 18% PEG-3350, 0.1 M TRIS pH 7.5 |
