6PXK

3.65 Angstroms resolution structure of HslU with an axial-channel plug

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9792
Spacegroup name	P 1 2 1
Unit cell lengths	200.030, 91.200, 201.800
Unit cell angles	90.00, 99.43, 90.00

Refinement procedure

Resolution	47.687 - 3.647
R-factor	0.2317
Rwork	0.230
R-free	0.27050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1do0
Data reduction software	HKL-2000
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.687	47.687	3.740
High resolution limit [Å]	3.647	16.310	3.650
Rmerge	0.105	0.042	1.287
Rmeas	0.117	0.047	1.440
Number of reflections	154279	1652	10641
<I/σ(I)>	8.9	30.18	1.02
Completeness [%]	98.4	92.9	92.5
Redundancy	5.01	4.85	4.689
CC(1/2)	0.998	0.997	0.609

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M ammonium sulfate, 18% PEG-3350, 0.1 M TRIS pH 7.5