6PXB

N-Terminal SH2 domain of the p120RasGAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 32
Unit cell lengths	64.021, 64.021, 119.447
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.816 - 1.747
R-factor	0.2221
Rwork	0.220
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k44
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.810
High resolution limit [Å]	1.747	3.770	1.750
Rmerge	0.080	0.062	0.835
Rmeas	0.084	0.065	0.890
Rpim	0.026	0.020	0.303
Number of reflections	55502	5536	5568
<I/σ(I)>	5.4
Completeness [%]	99.9	100	99.9
Redundancy	9.9	10.4	8
CC(1/2)		0.999	0.909

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	21% PEG 10,000 0.1M Tris pH 8.0 0.2M Ammonium Acetate