6PVW
RNase A in complex with cp4pA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER IMUS MICROFOCUS |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-30 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.640, 32.710, 72.680 |
| Unit cell angles | 90.00, 90.57, 90.00 |
Refinement procedure
| Resolution | 25.150 - 1.600 |
| R-factor | 0.1913 |
| Rwork | 0.189 |
| R-free | 0.22610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1afu |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.160 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.068 | 0.490 |
| Rmeas | 0.071 | 0.534 |
| Rpim | 0.021 | 0.208 |
| Number of reflections | 31680 | 3142 |
| <I/σ(I)> | 18.5 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.8 | 6.4 |
| CC(1/2) | 0.999 | 0.877 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 20 mM sodium citrate, 20% PEG 4000 |
