6PUN

Crystal structure of a ternary complex of FBF-2 with LST-1 (site B) and compact FBE RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	42.751, 74.380, 81.547
Unit cell angles	107.17, 104.40, 101.76

Refinement procedure

Resolution	42.691 - 2.099
R-factor	0.2001
Rwork	0.198
R-free	0.24040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v74
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.099	5.700	2.100
Rmerge	0.108	0.092	0.749
Rmeas	0.118	0.099	0.813
Rpim	0.045	0.038	0.312
Number of reflections	50128	2562	2514
<I/σ(I)>	9
Completeness [%]	96.9	99	97.1
Redundancy	6.6	7	6.5
CC(1/2)		0.990	0.865

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	17-20% w/v PEG3350, 0.2 M magnesium chloride, 0.1 M MES