6PTQ
Dark, Room Temperature, PCM Myxobacterial Phytochrome, P2, Wild Type
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SACLA BEAMLINE BL3 |
Synchrotron site | SACLA |
Beamline | BL3 |
Temperature [K] | 290 |
Detector technology | CCD |
Collection date | 2019-04-22 |
Detector | MPCCD |
Wavelength(s) | 1.38 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 83.690, 83.400, 86.870 |
Unit cell angles | 90.00, 107.63, 90.00 |
Refinement procedure
Resolution | 32.900 - 2.100 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | CrystFEL |
Data scaling software | CrystFEL |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.900 | 2.130 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 58755 | 2518 |
<I/σ(I)> | 4.8 | |
Completeness [%] | 100.0 | |
Redundancy | 573.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | Ammonium acetate, Sodium citrate tribasic dihydrate, PEG 4000, Benzamidine Hydrochloride |