6PTA
Crystal structure of the ARF family small GTPase ARF1 from Candida albicans in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2019-04-08 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 86.801, 42.285, 90.343 |
| Unit cell angles | 90.00, 93.70, 90.00 |
Refinement procedure
| Resolution | 24.500 - 2.500 |
| R-factor | 0.2594 |
| Rwork | 0.257 |
| R-free | 0.29480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r8q |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.782 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15_3448) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.118 | 0.834 |
| Rpim | 0.076 | 0.533 |
| Number of reflections | 22742 | 1081 |
| <I/σ(I)> | 8.61 | 1.02 |
| Completeness [%] | 98.5 | 97 |
| Redundancy | 3.2 | 3.2 |
| CC(1/2) | 0.798 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 0.2 M ammonium Acetate, 30% PEG 4K, 0.1M sodium acetate pH 4.6 |
