6PSE
Complex of BICD2 with a Dynein Light Intermediate Chain Peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-08 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97750 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.773, 61.844, 81.681 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.080 - 2.404 |
R-factor | 0.1955 |
Rwork | 0.193 |
R-free | 0.24660 |
Structure solution method | SAD |
Data reduction software | HKL-2000 (v701.c3) |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.12_2829) |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.040 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.109 | 0.785 |
Rpim | 0.033 | 0.760 |
Number of reflections | 19602 | 1396 |
<I/σ(I)> | 12 | |
Completeness [%] | 94.9 | |
Redundancy | 11.2 | |
CC(1/2) | 0.990 | 0.289 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 16% v/v 2-propanol, 0.08M Sodium citrate tribasic dehydrate, pH 5.5, 18% w/v polyethylene glycol 4,000 |