6PRG

SBP RafE in complex with stachyose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-002
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-21
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54187
Spacegroup name	P 21 21 21
Unit cell lengths	49.278, 118.839, 146.012
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.940 - 2.350
R-factor	0.2239
Rwork	0.223
R-free	0.24680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2i58
RMSD bond length	0.002
RMSD bond angle	0.677
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.390
High resolution limit [Å]	2.350	2.350
Rmerge	0.084	0.181
Rpim	0.036	0.095
Number of reflections	36605	3742
<I/σ(I)>	20.2
Completeness [%]	99.9
Redundancy	5
CC(1/2)	0.995	0.966

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	0.2M CsCl, 0.1M Tris, 20% w/v PEG 3350