6POP
Crystal structure of DauA in complex with NADP+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-14 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.687, 232.817, 88.257 |
| Unit cell angles | 90.00, 119.18, 90.00 |
Refinement procedure
| Resolution | 39.853 - 2.147 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.26020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6pnu |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.853 | 2.180 |
| High resolution limit [Å] | 2.147 | 2.150 |
| Rmerge | 0.136 | 0.579 |
| Rpim | 0.072 | 0.328 |
| Number of reflections | 167353 | 8002 |
| <I/σ(I)> | 9 | |
| Completeness [%] | 99.2 | 98.1 |
| Redundancy | 5.4 | |
| CC(1/2) | 0.989 | 0.671 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 0.2 M sodium acetate trihydrate, 0.1 M Bis-Tris and 20% PEG 3350 |
