6PNY
X-ray Structure of Flpp3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE CXI |
| Synchrotron site | SLAC LCLS |
| Beamline | CXI |
| Temperature [K] | 300 |
| Detector technology | PIXEL |
| Collection date | 2015-04-02 |
| Detector | CS-PAD CXI-1 |
| Wavelength(s) | 1.425 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 28.000, 51.900, 68.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.000 - 1.650 |
| R-factor | 0.1994 |
| Rwork | 0.198 |
| R-free | 0.23490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2mu4 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.584 |
| Data reduction software | iMOSFLM |
| Data scaling software | CrystFEL |
| Phasing software | MR-Rosetta |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 16570 | 1143 |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 100.0 | |
| Redundancy | 217 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 300 | Sodium Chloride, Calcium Chloride, Sodium Phosphate |
