6PMP
Crystal structure of a fragment of rat phospholipase Cepsilon EF3-RA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2018-11-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.572, 127.755, 139.337 |
| Unit cell angles | 90.00, 101.12, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.730 |
| R-factor | 0.23591 |
| Rwork | 0.234 |
| R-free | 0.27278 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3OHM |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.714 |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 136.720 | 136.720 | 2.880 |
| High resolution limit [Å] | 2.730 | 8.640 | 2.730 |
| Rmerge | 0.268 | 0.049 | 1.794 |
| Rmeas | 0.319 | 0.058 | 2.120 |
| Rpim | 0.171 | 0.031 | 1.122 |
| Total number of observations | 288747 | 9194 | 43223 |
| Number of reflections | 84933 | 2783 | 12359 |
| <I/σ(I)> | 5.1 | 14.9 | 1.1 |
| Completeness [%] | 99.6 | 99.5 | 99.7 |
| Redundancy | 3.4 | 3.3 | 3.5 |
| CC(1/2) | 0.971 | 0.996 | 0.334 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 285.15 | 6.25% PEG 4000, 100 mM MES pH 6.00, 0.2 M NaCl |
