6PLN

X-ray crystal structure of Pyrococcus furiosus general transcription factor TFE-alpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-05
Detector	ADSC QUANTUM 210
Wavelength(s)	0.977
Spacegroup name	P 41 21 2
Unit cell lengths	55.689, 55.689, 248.673
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.270 - 2.600
R-factor	0.2444
Rwork	0.239
R-free	0.28650
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.125
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.064	0.991
Number of reflections	13000	645
<I/σ(I)>	59	3.55
Completeness [%]	99.9	100
Redundancy	13.1	12.8
CC(1/2)	0.842	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	10 % PEG 8000, 0.2 M MgCl2, and 0.1 M Tris-HCl (pH 7)