6PLL

Crystal structure of the ZIG-8 IG1 homodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	120
Detector technology	CCD
Collection date	2019-04-13
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9791928
Spacegroup name	P 65 2 2
Unit cell lengths	73.560, 73.560, 270.631
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.381 - 2.693
R-factor	0.2385
Rwork	0.237
R-free	0.26950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6on9
RMSD bond length	0.005
RMSD bond angle	0.753
Data reduction software	XDS (20190315)
Data scaling software	XDS (20190315)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.860
High resolution limit [Å]	2.690	2.690
Rmerge	0.225	2.597
Rmeas	0.230	2.673
Number of reflections	12889	1967
<I/σ(I)>	15.33	1.05
Completeness [%]	99.7	98.3
Redundancy	21.4	17.7
CC(1/2)	0.998	0.529

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.2 M Ammonium dihydrogen phosphate, 20% (w/v) PEG 3350