6PKZ
Structure of human DNA polymerase beta N279A complexed with 8OA in the template base paired with incoming non-hydrolyzable GTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2015-10-01 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.609, 78.555, 54.776 |
Unit cell angles | 90.00, 106.83, 90.00 |
Refinement procedure
Resolution | 19.640 - 2.740 |
R-factor | 0.178 |
Rwork | 0.175 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3isb |
RMSD bond length | 0.011 |
RMSD bond angle | 1.491 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.800 |
High resolution limit [Å] | 2.740 | 2.740 |
Rmerge | 0.126 | |
Number of reflections | 11337 | 509 |
<I/σ(I)> | 13.5 | |
Completeness [%] | 97.4 | |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 14-23% PEG3400, 350 MM SODIUM ACETATE IN 50 MM IMIDAZOLE, PH 7.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 295K |