6PKH

Zebrafish N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) catalytic domain auto-inhibited by pro-peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	73.954, 73.954, 109.288
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.026 - 1.600
R-factor	0.1399
Rwork	0.138
R-free	0.17260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pkg
RMSD bond length	0.007
RMSD bond angle	0.915
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Number of reflections	38530	2264
<I/σ(I)>	47.6
Completeness [%]	93.8
Redundancy	15.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	sodium citrate, HEPES pH 7.5, cryo-protected with sodium malonate