6PKF
Myocilin OLF mutant N428E/D478K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.506, 58.688, 89.505 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.369 - 1.484 |
R-factor | 0.152 |
Rwork | 0.150 |
R-free | 0.18090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ou1 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.369 | 1.540 |
High resolution limit [Å] | 1.480 | 1.480 |
Rmeas | 0.129 | |
Rpim | 0.039 | |
Number of reflections | 40126 | 41877 |
<I/σ(I)> | 31.73 | |
Completeness [%] | 97.6 | 95.49 |
Redundancy | 11.1 | |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | PEG 3350, M Bis Tris pH 6, magnesium formate |