6PJ9
Time-resolved structural snapshot of proteolysis by GlpG inside the membrane
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-20 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 71.260, 99.000, 63.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.840 - 2.500 |
| R-factor | 0.2587 |
| Rwork | 0.258 |
| R-free | 0.28070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5f5d |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.473 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.840 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 7975 | 871 |
| <I/σ(I)> | 4.5 | |
| Completeness [%] | 99.2 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Na-acetate pH 5.5, 3 M NaCl, and 5 % ethylene glycol |
