6PIL

Antibody scFv-M204 monomeric state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.7749
Spacegroup name	P 41 2 2
Unit cell lengths	60.420, 60.420, 159.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.500 - 2.200
R-factor	0.198
Rwork	0.194
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.150
Data reduction software	XDS
Data scaling software	XSCALE (20180126)
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	56.500	56.500	2.260
High resolution limit [Å]	2.200	9.840	2.200
Rmerge	0.072	0.022	0.644
Rmeas	0.084	0.027	0.752
Total number of observations	55416
Number of reflections	15422	220	1120
<I/σ(I)>	11.14	32.34	1.91
Completeness [%]	97.9	94.4	99
Redundancy	3.593	3.109	3.653
CC(1/2)	0.999	1.000	0.736

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	298	0.2 M Lithium sulfate, 0.1 M Phosphate/Citrate pH 4.2, 20% PEG 1000