6PII
The evolving story of AtzT, a periplasmic binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.008020 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 98.920, 63.050, 120.436 |
| Unit cell angles | 90.00, 93.49, 90.00 |
Refinement procedure
| Resolution | 49.400 - 1.870 |
| R-factor | 0.15624 |
| Rwork | 0.155 |
| R-free | 0.18303 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.548 |
| Data reduction software | ADDREF |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.400 | 1.900 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.140 | 0.669 |
| Rpim | 0.032 | 0.246 |
| Number of reflections | 119947 | 4833 |
| <I/σ(I)> | 13.5 | 3.1 |
| Completeness [%] | 97.8 | 80.1 |
| Redundancy | 19.6 | 7.4 |
| CC(1/2) | 0.998 | 0.809 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Protein at 20 mg/mL in SD2 plates incubated at 20 C. Conditions contained 6-8% Jeffamine M600 with 2-7% MPD and trisodium citrate at 1.1-1.2 M |
