6PIA
Crystal structure of Marinobacter subterrani acetylpolyamine amidohydrolase (msAPAH) complexed with 6-[(3-aminopropyl)amino]-N-hydroxyhexanamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.744, 163.170, 65.744 |
| Unit cell angles | 90.00, 94.44, 90.00 |
Refinement procedure
| Resolution | 46.270 - 1.750 |
| R-factor | 0.1765 |
| Rwork | 0.175 |
| R-free | 0.21160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB: 4ZUM |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.839 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.270 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rpim | 0.085 | 0.470 |
| Number of reflections | 131376 | 12851 |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 95.6 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 10 mg/ml msAPAH Protein, 0.2 M calcium chloride dihydrate, 20% w/v PEG 3350, 1:1 ratio protein to precipitant |
