6PHH

Unliganded human transmission blocking antibody 2544

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97949
Spacegroup name	C 1 2 1
Unit cell lengths	172.530, 43.650, 138.570
Unit cell angles	90.00, 111.22, 90.00

Refinement procedure

Resolution	38.976 - 2.400
R-factor	0.2192
Rwork	0.218
R-free	0.24870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	in house model
RMSD bond length	0.002
RMSD bond angle	0.551
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.131	0.724
Rpim	0.055	0.298
Number of reflections	38316	4305
<I/σ(I)>	11.2	1.6
Completeness [%]	99.9	100
Redundancy	6.7	6.9
CC(1/2)	0.996	0.549

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1 M sodium cacodylate HCl, pH 6.5, 1.0 M sodium citrate tribasic