6PHF
Pfs25 in complex with the human transmission blocking antibody 2587
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-03 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 85.236, 110.266, 147.097 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.752 - 3.100 |
| R-factor | 0.2386 |
| Rwork | 0.237 |
| R-free | 0.26650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | in house model |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.500 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.200 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.284 | 0.784 |
| Rpim | 0.119 | 0.334 |
| Number of reflections | 25850 | 2305 |
| <I/σ(I)> | 6.8 | 1.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.6 | 6.5 |
| CC(1/2) | 0.979 | 0.543 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.2 M potassium thiocyanate, 20% (w/v) PEG 3350, and cryoprotected in 15% (v/v) ethylene glycol |
