6PHF

Pfs25 in complex with the human transmission blocking antibody 2587

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97949
Spacegroup name	P 2 21 21
Unit cell lengths	85.236, 110.266, 147.097
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.752 - 3.100
R-factor	0.2386
Rwork	0.237
R-free	0.26650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	in house model
RMSD bond length	0.002
RMSD bond angle	0.500
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.200
High resolution limit [Å]	3.100	3.100
Rmerge	0.284	0.784
Rpim	0.119	0.334
Number of reflections	25850	2305
<I/σ(I)>	6.8	1.4
Completeness [%]	99.9	100
Redundancy	6.6	6.5
CC(1/2)	0.979	0.543

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.2 M potassium thiocyanate, 20% (w/v) PEG 3350, and cryoprotected in 15% (v/v) ethylene glycol