6PGV

Human Josephin-2 in complex with ubiquitin

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-23
Detector	ADSC QUANTUM 210
Wavelength(s)	0.900, 0.9787, 0.9792, 0.9700, 1.0053, 1.0089
Spacegroup name	P 61 2 2
Unit cell lengths	102.120, 102.120, 92.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.815 - 2.300
Rwork	0.201
R-free	0.22400
Structure solution method	MAD
RMSD bond length	0.002
RMSD bond angle	0.498
Data reduction software	XDS (Jan 26, 2018)
Data scaling software	XSCALE (Jan 26, 2018)
Phasing software	PHENIX ((1.14_3260))
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.058	0.990
Rmeas	0.058	1.000
Rpim	0.007	0.145
Number of reflections	12866	1237
<I/σ(I)>	81.6
Completeness [%]	98.3	97.4
Redundancy	77	46.2
CC(1/2)	1.000	0.949

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	4.6	293	0.1 M sodium acetate pH 4.6, 0.2 M CaCl2, 22.5% (w/v) PEG 6000; microbatch under Al's Oil