6PGT
Protein Tyrosine Phosphatase 1B (1-301), T177A mutant, vanadate bound state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2016-07-24 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.54 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 88.078, 88.078, 103.651 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.140 - 2.200 |
R-factor | 0.1903 |
Rwork | 0.188 |
R-free | 0.23010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qkq |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.140 | 2.279 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.086 | |
Rmeas | 0.090 | |
Rpim | 0.028 | |
Number of reflections | 23137 | 2296 |
<I/σ(I)> | 16.93 | |
Completeness [%] | 96.1 | |
Redundancy | 10.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1M Hepes, 0.2 M magnesium acetate and 15-20% polyethylene glycol 8000 |