6PGQ

Crystal structure of N-glycosylated human calcitonin receptor extracellular domain in complex with salmon calcitonin (22-32)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2017-08-09
Detector	RAYONIX MX300-HS
Wavelength(s)	0.97872
Spacegroup name	P 41 3 2
Unit cell lengths	181.288, 181.288, 181.288
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.450 - 2.850
R-factor	0.1942
Rwork	0.193
R-free	0.22460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.832
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.850	2.850
Number of reflections	24107	1167
<I/σ(I)>	21.24
Completeness [%]	99.0
Redundancy	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.7	293	1.94M Ammonium sulfate, 0.1M Sodium acetate, 15% Ethylene glycol