6PGE
WDR5delta32 bound to ethyl 3-(4-(hydroxymethyl)-1H-imidazol-2-yl)propanoate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-02-09 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.722, 65.180, 97.071 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.390 - 1.760 |
| R-factor | 0.18379 |
| Rwork | 0.182 |
| R-free | 0.21263 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6pg4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.414 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.720 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Number of reflections | 30209 | 1647 |
| <I/σ(I)> | 13.4 | 2.9 |
| Completeness [%] | 99.7 | 97.5 |
| Redundancy | 7.1 | 7 |
| CC(1/2) | 0.997 | 0.875 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 281 | 0.024 M ammonium sulfate, 0.1 M Bis-Tris chloride, pH 5.64, 26.3% w/v PEG4000 |
