6PG9
WDR5delta23 bound to N-(4-(5-(hydroxymethyl)-1H-imidazol-2-yl)butyl)benzamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-08-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.915, 61.493, 64.757 |
| Unit cell angles | 110.56, 91.06, 112.38 |
Refinement procedure
| Resolution | 52.420 - 1.750 |
| R-factor | 0.23435 |
| Rwork | 0.233 |
| R-free | 0.26877 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6pg3 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.430 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.420 | 1.790 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 62036 | 3516 |
| <I/σ(I)> | 4.4 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.7 | 3.8 |
| CC(1/2) | 0.970 | 0.781 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 281 | 0.182 M ammonium fluoride, 0.1 soidum cacodylate (pH 5.79), 22.1 %w/v PEG8000 |
