6PG8
WDR5delta23 bound to (2-(3-phenylpropyl)-1H-imidazol-4-yl)methanol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.118, 75.831, 79.239 |
| Unit cell angles | 104.70, 96.41, 105.23 |
Refinement procedure
| Resolution | 46.710 - 1.670 |
| R-factor | 0.18782 |
| Rwork | 0.187 |
| R-free | 0.20678 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6pg3 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.407 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.710 | 1.700 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Number of reflections | 128452 | 6091 |
| <I/σ(I)> | 11.3 | 2 |
| Completeness [%] | 96.4 | 92.1 |
| Redundancy | 3.8 | 3.8 |
| CC(1/2) | 0.999 | 0.751 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 281 | 0.179 M ammonium sulfate, 0.1 M bis-tris chloride (pH 5.62), 28.6 %w/v PEG3350 |
