6PFQ
Structure of Kluyveromyces marxianus Usb1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-21 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.927, 107.364, 113.902 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.593 - 1.800 |
R-factor | 0.1936 |
Rwork | 0.192 |
R-free | 0.22310 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.177 |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 107.360 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 80145 | 4528 |
<I/σ(I)> | 16.3 | |
Completeness [%] | 100.0 | |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 1.2 M potassium sodium tartrate, 100 mM HEPES, 30% glycerol |