6PEF
Vaccine-elicited NHP FP-targeting antibody DF2F-a.01 in complex with HIV fusion peptide (residue 512-519)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-12 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.574, 52.504, 151.716 |
Unit cell angles | 90.00, 90.68, 90.00 |
Refinement procedure
Resolution | 43.171 - 2.004 |
R-factor | 0.1845 |
Rwork | 0.183 |
R-free | 0.21360 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
Rmerge | 0.111 | 0.055 | 0.665 |
Rmeas | 0.131 | 0.065 | 0.820 |
Rpim | 0.068 | 0.034 | 0.473 |
Total number of observations | 263243 | ||
Number of reflections | 75515 | 3993 | 3662 |
<I/σ(I)> | 8.6 | ||
Completeness [%] | 97.9 | 98.5 | 96.1 |
Redundancy | 3.5 | 3.6 | 2.6 |
CC(1/2) | 0.994 | 0.575 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M NaCl, 0.1 M BIS-TRIS pH 6.5, 25% PEG 3350 |