6PDX
Crystal structure of C585 Fab in complex with influenza virus hemagglutinin from A/Switzerland/9715293/2013 (H3N2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-21 |
| Detector | RAYONIX MX425-HS |
| Wavelength(s) | 1.068 |
| Spacegroup name | P 63 |
| Unit cell lengths | 287.265, 287.265, 182.933 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.877 - 3.993 |
| R-factor | 0.2136 |
| Rwork | 0.212 |
| R-free | 0.24150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.830 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.700 | 4.090 |
| High resolution limit [Å] | 3.993 | 3.993 |
| Number of reflections | 71845 | 4312 |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 99.7 | 97.5 |
| Redundancy | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 0.1M sodium cacodylate, 1M sodium citrate |
