6PDL

Crystal Structure of Hendra Virus Attachment G Glycoprotein in Complex with Receptor Ephrin-B2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-06-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1
Unit cell lengths	75.764, 94.642, 102.522
Unit cell angles	112.86, 97.44, 97.56

Refinement procedure

Resolution	37.561 - 2.700
R-factor	0.1746
Rwork	0.172
R-free	0.22690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3d11
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.800
High resolution limit [Å]	2.700	5.810	2.700
Rmerge	0.076	0.046	0.207
Total number of observations	241203
Number of reflections	65721	6984	5103
<I/σ(I)>	10.1
Completeness [%]	93.8	99.4	73.3
Redundancy	3.7	3.8	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	27.5% PEG2000MME and 0.09% MG7