6PCO
Mechanism for regulation of DNA binding of Bordetella bronchiseptica BpsR by 6-hydroxynicotinic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-08 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.870, 90.523, 103.051 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.030 - 2.750 |
| R-factor | 0.23479 |
| Rwork | 0.231 |
| R-free | 0.30139 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nnn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.217 |
| Data reduction software | HKL-3000 (717.1) |
| Data scaling software | HKL-3000 (717.1) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.030 | 2.850 |
| High resolution limit [Å] | 2.650 | 2.750 |
| Rmerge | 0.058 | 0.549 |
| Rmeas | 0.068 | 0.642 |
| Rpim | 0.034 | 0.330 |
| Number of reflections | 17964 | 1712 |
| <I/σ(I)> | 23.4 | 4.38 |
| Completeness [%] | 98.7 | 97.44 |
| Redundancy | 1.1 | 1.1 |
| CC(1/2) | 0.999 | 0.906 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 285 | 18% PEG 2250, 0.2 M potassium formate, and 15% butanediol |
