6PBS
Structure of ClpC1-NTD in complex with Ecumicin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.063, 130.342, 112.562 |
| Unit cell angles | 90.00, 90.07, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.500 |
| Rwork | 0.180 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6pba |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.075 | 0.638 |
| Rmeas | 0.098 | 0.786 |
| Rpim | 0.041 | 0.364 |
| Number of reflections | 79421 | 3828 |
| <I/σ(I)> | 21.5 | |
| Completeness [%] | 98.8 | |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.2 M calcium acetate hydrate, 0.1 M Tris, pH 7.0, 20% PEG3000 |
